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簡要描述:CCL-229 LoVo 人結(jié)腸癌細(xì)胞 atcc 細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!
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CCL-229 LoVo 人結(jié)腸癌細(xì)胞
ATCC® Number: CCL-229™
Designations: LoVo
Depositors: M Romsdahl
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: colon
Tumor Stage: Dukes' type C, grade IV
Disease: colorectal adenocarcinoma
Derived from metastatic site: left supraclavicular region
Cellular Products: carcinoembryonic antigen (CEA) 908 ng/10 exp6 cells/10 days
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CCL-229 LoVo 人結(jié)腸癌細(xì)胞
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic: Yes
Reverse Transcript: negative
Oncogene: myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src -
Antigen Expression: HLA A11, B15, B17, Cw1, Cw3; blood type B
DNA Profile (STR): Amelogenin: XY
CSF1PO: 10,11,13,14
D13S317: 8, 11
D16S the colon. [1049]
The cells are negative for expression of CSAp (CSAp-) and colon antigen 3.
The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. [22861]
Myb, and fos oncogenes. [22861]
N-myc and sis oncogene expression were not detected. [22861]
Pr20
References: 1047: Drewinko B, et al. Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line. J. Natl. Cancer Inst. 61: 75-83, 1978. PubMed: 276641
1048: Drewinko B, Yand LY. Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells. Exp. Cell Res. 101: 414-416, 1976. PubMed: 964319
1049: Drewinko B, et al. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res. 36: 467-475, 1976. PubMed: 1260746
22861: Trainer DL, et a l. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
23341: Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905
26057: Drewinko B, et al. Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives. Cancer Res. 39: 2630-2636, 1979. PubMed: 445465
32913: Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538
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